HPLC Quantitation For Assay and Chromatographic Purities

Raw material to be determined is dissolved in an organic solvent and automatically injected into the column of separation where the column may be normal or reverse phase mechanism. The separation carrier is a liquid solvent. The retention time and the peak response of raw material is compared to the known reference standard. Based on the peak response, the potency of the active ingredients can be calculated. The detector can be UV, fluorescence, refractive index or conductivity, etc.

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